원문정보
초록
영어
Plants can communicate each other through common underground mycorrhizal networks (CMNs). Through this research, we show that mycorrhizal networks can act as a conduit for signals that affects plant’s immune response. We set the mycorrhizal connections as an operational factor and examined whether plants would have enhanced immune response by communicating with other plants that had been exposed to pathogen. To do this, we compared plants that were connected with other plants with plants that have no such connections. We examined Phenylalanine ammonia-lyase (PAL) activity of each plant groups when we treated Tobacco Mosaic Virus (TMV) as a pathogen. PAL is an important defensive enzyme of plants that changes L-phenylalanine into trans-cinnamic acid and NH4 +. The higher PAL activity is the stronger immune response occurs. The experiments showed that the connected group has higher PAL activity than the non-connected group, which indicates that there might be a possible relationship between interplant communication via common mycorrhizal networks (CMNs) and enhanced immune response.
목차
1. INTRODUCTION
2. THEORETICAL BACKGROUND
2.1. Arbuscular mycorrhizas
2.2. PAL (Phenylalanine ammonia-lyase)
3. MATERIALS AND METHODS
3.1. Experimental design
3.2. TMV Inoculation
3.3. Calibration curves
3.4. PAL extraction and Assay
4. RESULTS
4.1. Calibration curve
4.2. PAL activity
5. DISCUSSION
6. CONCLUSION
7. REFERENCES
8. APPENDIX