원문정보
초록
영어
As information technology is developing, the Smart phone became one of the ways to spread diseases. However, most people still do not care about contamination from Smart phones. At least, some are using antibacterial coating to cleanse the liquid crystal display, but these antibacterial coatings have latent dangers especially the nano materials. If silver accumulates in the body, it doesn’t exit easily even if people use hemodialysis and heavy metal removal. Therefore the goal of this research is to make safe antibacterial smart coatings. This research will focus on Quorum Quenching effect of onion and garlic. Quorum Quenching inhibits the signaling responses include adaptation to availability of nutrients, defense against other microorganisms which may compete for the same nutrients and the avoidance of toxic compounds potentially dangerous for the bacteria. It is very important for pathogenic bacteria during infection of a host to co-ordinate their virulence in order to escape the immune response of the host in order to be able to establish a successful infection. In this research, we used Escherichia coli, Staphylococcus aureus, Salmonella enteric, which are three representative bacteria that inhabits in Smart phones. Experiments were conducted using Congo Red staining method and Crystal violet staining method.
목차
1. INTRODUCTION
1.1. Quorum Sensing
1.2. Effects of Quorum Quenching
1.3. Types of signal molecules
2. MATERIALS
2.1. Bacterial culture
2.2. In this research, 3 types of mediums were used, namely:
2.3. Culture condition
2.4. Concentrating method of onion and garlic
2.5. Experimental machines and equipment
3. METHODS
3.1. Congo Red1 solid medium and streaking
3.2. Congo Red solid medium with higher concentration of onion and garlic
3.3. Verify the red part and black part of Congo Red solid medium
3.4. Using Cyrstal Violet to compare the amount of biofilm formation
4. RESULTS
4.1. Comparing biofilm formation and sterilization effect by using Congo Red solid medium
4.2. Investigation of red and black parts of Congo Red solid mediums
4.3. Using Crystal Violet to compare the amount of biofilm formation
5. CONCLUSION AND DISCUSSION
6. ACKNOWLEDGEMENTS
7. REFERENCES