earticle

영어

Cell cryopreservation plays a central role in reproductive technology. A major impediment to the development of cell cryopreservation is that there has yet to be found cryopreservation solutions that successfully prevent destruction of cellular tissue caused by intracellular hydro expansion. This study utilized the Korean traditional bioceramic onggi in sperm cryopreservation. Onggi emits far-infrared ray that stimulates cellular biological activities, promotes antioxidant effects, and prevents cellular toxicity. This study explored onggi’s ability to maintain the structural integrity and motility of sperm when onggi exposure media are used as cryopreservation solutions. It also tested whether onggi exposure media could alleviate freeze damage caused by ice crystals and reduce toxicity of cryoprotectants. The results showed that onggi exposure media reduced sperm toxicity during freezing, minimized abrupt thermal changes during thawing, and promoted sperm motility before and after actual cryopreservation. Onggi exposure media could also alleviate the damaging toxicity effect that existing cryoprotectants inherently have on cells. In conclusion, if the conditions to produce a synergy effect between onggi and cryoprotectants during cryopreservation are met, the newfound properties of onggi may well aid in the advance of reproductive technology