earticle

영어

Nhp6A, and Nhp6B in Saccharomyces cerevisiae are homologues with Human Hmgb1 which belongs to High Mobility Group Box (HMGB) family. Hmgb1 is believed to play an important role in autophagy and apoptosis. Besides the roles in necrosis, however, the exact roles of Nhp6A and Nhp6B are not studied so far. Recently, it is suggested that Nhp6A also plays important roles in autophagic cell death of S. cerevisiae. Our study is to assess the roles of Nhp6A, Nhp6B, and HMGB1 in overexpression of cell death of S. cerevisiae under various stress conditions. We did transformation of the genes HMGB1 and NHP6A into S. cerevisiae BY4743 strain and its mutants (NHP6A-/-, NHP6B-/-, NHP6A/B-/-). Using these strains, we compared the growth rate and colony formation under various chemical stress factors-EDTA, D-sorbitol, acetic acid, methyl methane sulfonate, dithiothreitol, NaCl, 4-nitroquinoline 1-oxide. Under dithiothreitol, BY4743-HMGB1 shows high viability, while BY4743-NHP6A shows low viability. In contrast, under acetic acid, BY4743-HMGB1 shows low viability, while BY4743-NHP6A shows high viability. These results imply that HMGB1 plays important roles in dithiothreitol(ER stress) induced cell death and acetic acid(pH stress) induced cell death.