earticle

영어

To find out the reason why some protein which has specific sequence was not dyed by CBB that is commonly used as dying solution. This can be identify purification of protein like p53Transactivation Domain (TAD) which is not dyed by CBB and help the research of this proteins. After refinement, we're going to figure out through SDS-PAGE why separated TAD protein can't be dyed itself CBB and look for its solution. After finding out a dyeing principle of CBB, we can figure out the reason for not being dyed through analyzing a sequence of TAD. And we find another dying method that in accordance with TAD sequence. We can find that TAD is finally dyed after trying the method of silver staining. The reason why TAD is not dyed by CBB is there is a few amino acids that bind with CBB. We dyed TAD using silver staining that bind with another amino acids in TAD sequence.