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Background: To evaluate the effect of Kinesio taping applied on the ankle instability, joint range of motion and balance. Methods: The participants included in this study were male and female, 20~30 ages, who experiencing an ankle sprain or had chronic pain, did not exercise during the intervention, and did not experience severe exercise at least 3 weeks before. A total of twenty-four participants were divided into two groups: Kinesio taping applied group (n=12) and control group (n=12). The experiment was conducted for a three days. Measurements were taken for ankle joint range of motion using goniometer, and measurements were taken for balance using good balance system. Pre– test measurements were conducted on before Kinesio taping apply, and 24 hours after, 48 hours after, 72 hours after measurements were conducted. Statistical analysis was done using a independent samples t-test and repeated measure ANOVA. Results: There were significant differences to the duration of intervention in ankle joint range of motion and balance within the both group. However, there was a significant differences Kinesio taping group when comparing the groups. Conclusions: According to the results of this study, applying Kinesio taping to ankle instability is more effective on ankle joint range of motion and to recover balance.