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Bio-Controlling Effect of Oyster Shell on Bacteria in Polluted Soil and Water in Seaside Areas

원문정보

Yoo Bin Shin

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초록

영어

Water pollution is an alarming environmental problem. One of the largest unknown causes of ocean water pollution is the minimally used oyster shell that was wrongfully disposed of. Therefore, oyster shell waste was used to understand the degree of its effects on water pollution. In doing so, seawater and sea soil obtained from less-polluted and more-polluted areas of the Gwanganlli shore were cultivated with oyster shell or its extract. To measure the rate of bacterial growth, oyster shells were grinded into different sizes then extracted to cultivate bacteria. The growth rate was tested by measuring their absorbance. Finally to test whether oyster shell effectively suppress growth of malevolent bacteria, oyster shell extract was cultivated with 5 different malevolent bacteria. The results show that oyster shell extract is more effective in preventing the growth of bacteria collected from seawater than those collected from the soil. Specifically, oyster shell extract powder greater than 500um most effectively suppressed bacterial growth, but did not selectively inhibit the growth of malevolent bacteria caused by water pollution, instead inhibited entire growth. Moreover, >500um extract 5% solution effectively suppressed the growth of malevolent bacteria. Thus, spraying oyster shell or its extract on polluted shores can effectively suppress the growth of malevolent bacteria.

목차

ABSTRACT
 1. INTRODUCTION
 2. MATERIALS AND METHODS
  Preparation of Bacteria Culture Media
  Collecting of polluted soil and seawater
  Preparation of Oyster Shell powder and extract
  Classification of Bacterial Colonies and Measurement of Absorbance
  Growth of Bacteria from Polluted Areas
  Effect of OS on Growth of Bacteria from Polluted Areas
  Growth of Bacteria Cultured with Different Concentrations of OS
  Growth of Bacteria Cultured with Different Size OS concentrates
  Effects of OS Extract Concentration on Bacterial Growth
 3. RESULTS
  Bacterial Diversity in Polluted Areas
  Effects of OS on Bacterial Growth
  Effects of OS Concentration on Bacterial Growth
  Growth of Bacteria Cultured with Different Size OS Powders
  Growth of Harmful Bacteria Cultured with Different Concentrations and Sizes of OS
  Effects of Different OS Size Extracts and Concentration on Harmful Bacteria
 4. DISCUSSION AND CONCLUSION
 5. REFERENCES

저자정보

  • Yoo Bin Shin Korea International School

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