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초록
영어
As a result of culturing E.Coli in undernutrition condition, while there was no change in general growth, two types of E.Coli, energy storing E.Coli which contains resistance to different stressing environment and energy consuming E.Coli with increased energy consumption and decreased resistance to each stress, was able to be separated. Their character could be inherited even after repeated culture, and this was confirmed throughout the experiment; it was mutation in a perspective of epigenetics. Energy storing E.Coli obtained increased resistance to low temperature, thus if this E.Coli was used to store gene and transform character, the efficiency increased.
목차
ABSTRACT
1. Introduction
2. Materials and Methods
Materials
Method
3. Results and Discussion
Preparation of Nutrient Agar Culture Medium and Nutrient Broth Culture Medium for Cultivation of E.Coli
Re-Cultivation of E.Coli Kept in Refrigeration
Dilution of NB Culture Medium to Specific Concentration
Incubation of E.Coli on the NB Culture Medium Diluted to ½, ¼, and 1/8 of Original Concentration
Reaction of E.Coli Samples, Cultivated in NB Culture Medium Diluted to ½, ¼, and 1/8 of its Original Concentration, to Antibiotics
Reaction of E.Coli Samples, Cultivated in NB Culture Medium Diluted to ½, ¼, and 1/8 of its Original Concentration, to Hydrogen
Bacteriophage Screening and Separation of Homogenous Bacteriophage
Reaction of E.Coli Samples, Cultivated in NB Culture Medium Diluted to ½, ¼, and 1/8 of its Original Concentration, to Bacteriophage Infection
Comparison of Energy Consumption in Energy Consuming type to Energy Storing Type of E. Coli
Comparison of Toxin Production in Energy Consuming type and Energy Storing Type of E.Coli
Transplant of Plasmid DNA, that Makes E.Coli Ampicillin-Tolerant, in Energy Saving E.Coli and Energy Consuming E.Coli
Storage Period of Antibiotics-Tolerant plasmid DNA Transplanted Energy Storing E.Coli and Energy Consuming E.Coli
4. Conclusion
5. Reference
1. Introduction
2. Materials and Methods
Materials
Method
3. Results and Discussion
Preparation of Nutrient Agar Culture Medium and Nutrient Broth Culture Medium for Cultivation of E.Coli
Re-Cultivation of E.Coli Kept in Refrigeration
Dilution of NB Culture Medium to Specific Concentration
Incubation of E.Coli on the NB Culture Medium Diluted to ½, ¼, and 1/8 of Original Concentration
Reaction of E.Coli Samples, Cultivated in NB Culture Medium Diluted to ½, ¼, and 1/8 of its Original Concentration, to Antibiotics
Reaction of E.Coli Samples, Cultivated in NB Culture Medium Diluted to ½, ¼, and 1/8 of its Original Concentration, to Hydrogen
Bacteriophage Screening and Separation of Homogenous Bacteriophage
Reaction of E.Coli Samples, Cultivated in NB Culture Medium Diluted to ½, ¼, and 1/8 of its Original Concentration, to Bacteriophage Infection
Comparison of Energy Consumption in Energy Consuming type to Energy Storing Type of E. Coli
Comparison of Toxin Production in Energy Consuming type and Energy Storing Type of E.Coli
Transplant of Plasmid DNA, that Makes E.Coli Ampicillin-Tolerant, in Energy Saving E.Coli and Energy Consuming E.Coli
Storage Period of Antibiotics-Tolerant plasmid DNA Transplanted Energy Storing E.Coli and Energy Consuming E.Coli
4. Conclusion
5. Reference
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