원문정보
초록
영어
S. pasteurii is a spore-forming nonpathogenic bacteria that is able to conduct bio-mineralization. Due the discovery of such a unique ability being recent, the possible applications of this characteristic are still mostly unexplored. While commercial applications of S. pasteurii as a cement reinforce does exist, there has been no exploration in applying this ability to improving water retention. Therefore, this study explored whether S. pasteurii’s ability to bio-mineralize could improve water retention ability of soil, while not harming the plants within the soil. Other soil bacteria were examined for the same criteria as well. The experiment had a few main procedures: examining the water retention ability of soil inoculated with these bacteria, measuring the chlorophyll and growth of cabbages grown in bacteria-infested soil, and measuring the viability of grass when inoculated with bacteria. The soil bacteria was identified and cultivated in TSA Media containing Urea. Only those who thrived within those conditions were further tested. S. pasteurii and the other bacteria were also cultivated in TSB Media with Urea. Adjusted to be at the same concentration, these were then inoculated in soil, which had cabbage seeds planted in it. The cabbages were then measured for length and chlorophyll A and B, while the soil’s water retention ability was tested by adding water in 1 ml increments and measuring how much leaked through. To measure the viability of the grass, bacterial solutions at the same concentration were added to identical patches of grass, which were then monitored for 9 days. S. pasteurii increased the soil retention ability the most, but also producing the lowest chlorophyll A and B content. Therefore, its plasmid DNA was transferred through heat shock transformation to soil bacteria d (Bacillus pumilus), which had shown positive signs to plant growth. The new bacterium was not harmful to plants, while also increasing the soil retention ability considerably. In conclusion, S. pasteurii indeed can be used to increase water retention in soil. However, due to its adverse effects on plants, creating possible hybrids to remove this defect is encouraged.
목차
1. INTRODUCTION
2. MATERIALS AND METHODS
2.1 Preparation of TSB Media:
2.2 Preparation of TSA Media:
2.3 Preparation of NA Media:
2.4 Preparation of NB Media:
2.5 Preparation of Skim Milk Media:
2.6 Preparation of Sporosarcina pasteurii
2.7 Selection of Urea-Resistant Soil Bacteria and DNA analysis
2.8 Draining Test for Soil with Soil bacteria and S. pasteurii
2.9 Effects of Soil Bacteria and S. pasteurii on growth of chinese cabbage in dry environments
2.10 Effects of Soil Bacteria on viability of grass in dry environments
2.11 Plasmid DNA Isolation of S. pasteurii
2.12 Heat shock transformation
2.13 Effects of transformed bacteria on plant growth and water retention ability
2.14 Application
3. RESULTS
3.1 Isolation of Urea-Resistant Soil Bacteria and S. pasteurii
3.2 Effects of S. pasteurii and soil bacteria on water retention of soil
3.3 DNA Analysis of Soil Bacteria
3.4 Effects of Soil Bacteria and S. pasteurii on growth of Chinese cabbage in dry environments
3.5 Effects of Soil Bacteria and S. pasteurii on viability of grass in dry environments
3.6 Effects of transformed bacteria on plant growth and water retention ability
4. DISCUSSION
5. CONCLUSION
6. References