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Oil spill is one of the major causes of pollution around the world. The tragic accident happened in Gulf of Mexico in 2010, which is considered as the worst oil spill in United States history. At this time, one ecologist found out the oil-degrading microorganism in this area and that it could become a very practical approach to oil spill cleanup. Sea water from 3 different sites of Qingdao Ocean is collected. The purpose was to see if there exist oil-degrading microbes in Qingdao Sea. First, after oil was put in Qingdao sea water for one week, it was verified that sea water in Qingdao has the ability to degrade oil. Then, to find out what degraded oil in the sea water in Qingdao, the water is isolated and cultivated. To further confirm it is oil-degrading microorganism which dissolves oil in Qingdao water, 4 beakers which contained crude oil, cultured microbes and 40ml of tap water, saturated soap water, and tap water were observed after 24 hours. When considering the percentage of remaining oil in crude oil as 100%, tap water with cultivated microbes degraded 27.41% of the oil, which is much higher data than in other solutions. Thus, it can be concluded that cultivated microorganism has great oil-degrading ability and it is those microorganism that dissolved oil in sea water in Qingdao. By further research, the type of microorganism found in Qingdao Ocean can be determined. Once it is determined, it will be more efficient and eco-friendly to use them in cleaning the oil with other physical methods.