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The number of collegiate females wearing heel shoes is ever increasing. Although high-heeled shoes offer shapely figures, they have been associated with instability and falling, leading to ankle injuries etc. Furthermore, high-heels negatively affect the normal lining of a female curvature, aggravating chronic lumbar diseases. Despite its significant importance, the number of researches on high-heels is limited and the effects of shoes-heel height haven’t been previously reported. Thus, the purpose of this study was to investigate the balance ability and ankle muscular function of female college students. In the present study, the effects of wearing of three types of shoes-heel height on static balance and isokinetic ankle muscular function were investigated. The 78 of habitual wearers of three different types shoes for 40.10 ± 10.12 weeks were recruited in this study (mean age: 22.60 ± 0.65 years). The participants were grouped by the shoe-heel height; low-heels group (LH, n=18), mid-heels group (MH, n=34), and high-heels group (HH, n=26). The heel height of three groups were 3.77 ± 1.69 cm (LH), 7.43 ± 0.53 cm (MH) and 9.50 ± 0.64 cm (HH), respectively. All participants were tested for their body composition and static balance ability. Also, their ankle joints were isokinetically tested for plantarflexion / dorsiflexion and eversion / inversion at 60d/s and 120d/s, respectively. Statistical analysis was conducted with the SPSS software (ver 21.0; SPSS Inc, Chicago, IL). All data are were reported as mean ± standard deviation. Prior to the comparison of measurements including body composition, static balance, and isokinetic torque variables, the descriptive statistics were calculated for all dependent variables. The among-group factor was the study groups (i.e. LH vs MH vs HH). Because the data for this study were not normally distributed, we analyzed the data using non-parametric tests. The Kruskal-Wallis rank test was conducted to identify differences among groups. The significance level for all analyses was set a priori at p ≤0.05. Our results of this present study may summarize as follow. First, compared to LH and MH groups, the dorsiflexor peak torque of HH was significantly higher in dominant and non-dominant ankles. Although the dorsiflexor work per repetition and total work of HH were higher than other two groups, only the variables of non-dominant ankle was significantly higher. The dorsiflexor fatigue index of HH showed the lower tendency compared to LH and MH groups. Second, compared to LH and MH groups, the plantarflexor peak torque of HH was significantly higher in dominant and non-dominant ankles. The plantarflexor work per repetition and total work of HH also were significantly higher than other two groups in both ankles. The plantarflexor fatigue indices of HH showed the lower tendency than those of both groups. Third, compared to LH and MH groups, the evertor peak torque of HH was significantly higher in dominant and non-dominant ankles. The evertor work per repetition and total work of HH showed the higher tendency than other two groups. Although the evertor fatigue index in dominant side of HH was significantly lower than those of both groups, that of non-dominant side showed the lower tendency compared to LH and MH. Last, compared to LH and MH, the invertor peak torque of HH represented the lower tendency in dominant and non-dominant ankles. The invertor work per repetition and total work of HH were significantly lower than those of other two groups except for work per repetition of non-dominant side. The invertor fatigue indices in both sides of HH showed the lower tendency compared to LH and MH. In conclusion, the results showed that the wearing of high-heels for short years had significantly increased isokinetic muscular functions (dorsiflexor, plantarflexor, evertor) of the ankles except for invertor compared to wearing of low or mid heels. We may confirm that the wearing of high-heels help to enhance the strength and endurance of the ankle joints and these results can affect to increase the static balance in female students under about 1 year.