한우의 말초혈액 단핵세포 염증성 사이토카인에 대한 브로피리민의 효과 확인


Identification of Inflammatory Cytokines in Bropirimine-Treated Peripheral Blood Mononuclear Cells from Hanwoo (Bos taurus coreanae)

김승창, 이승환, 채한화, 김의형, 정기용, 임다정, 박종은, 조용민, 최봉환

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Bropirimine, a class of antineoplastic agents, is known as one of the potent immunomodulators and is currently under clinical development for the treatment of cancer. However, the effect of bropirimine on the cow remains unknown as a therapeutics agent. In this experiment, the effect of bropirimine in the peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide (LPS) or concanavalin-A (Con-A) was examined. Jugular venous blood was collected from Korean Hanwoo calves and PBMCs were isolated. It was used to study the effect of bropirimine upon stimulation with LPS or Con-A for 72 hours. The expression pro-inflammatory cytokines like Tumor Necrosis Factor α (TNF-α) and Interferon γ (IFN-γ) were confirmed. Bropirimine significantly inhibited LPS- or Con-A-induced TNF-α and Con-A-induced IFN-γ in dose-dependent manner. Furthermore, Bropirimine inhibited TNF-α and Con-A mRNA expression at the transcription level. These results clearly indicated that bropirimine inhibited LPS or Con-A stimulated up-regulation of proinflammatory cytokines in a dose-dependent manner without conspicuous cytotoxicity. The bropirimine has potential to protect cow from LPS or Con-A induced endotoxin shock, possibly through inhibition of the production of proinflammatory cytokines. It suggesting that bropirimine may be a novel therapeutic agent for the prevention of inflammatory diseases. This result revealed specific features of the immune responses depending on the bropirimine compound and would help to knowledge of bovine immunity.


  Animals and materials
  Cell isolation, culture and stimulation
  Cell viability assay
  RNA isolation and cDNA synthesis
  Quantitative real time polymerase chain reaction(qRT-PCR) analysis
  Enzyme-linked immunosorbent assay (ELISA)
  Effects of bropirimine on the viability of PBMCs
  Effects of bropirimine on induced TNF-α and IFN-γ production by LPS or Con-A
  Effects of bropirimine on induced TNF-α and IFN-γ mRNA expression by LPS or Con-A


  • 김승창 Seungchang Kim. 국립축산과학원 동물유전체과
  • 이승환 Seung-Hwan Lee. 충남대학교 동물자원생명과학과
  • 채한화 Han-Ha Chai. 국립축산과학원 동물유전체과
  • 김의형 Ui-Hyung Kim. 한우연구소
  • 정기용 Ki-Yong Chung. 한우연구소
  • 임다정 Dajeong Lim. 국립축산과학원 동물유전체과
  • 박종은 Jong-Eun Park. 국립축산과학원 동물유전체과
  • 조용민 Yongmin Cho. 국립축산과학원 동물유전체과
  • 최봉환 Bong-Hwan Choi. 국립축산과학원 동물유전체과


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