earticle

영어

This study was performed to evaluate anti-fatigue effect of hydrogen water (HW) drinking in chronic forced exercise mice model. Twelve-week old C57BL6 female mice were separated into non-stressed normal control (NC) group, and stressed group: purified water (PW)-treated group and HW treated group. Stress groups were supplied with PW and HW respectively ad libitum, and forced to swim for the stress induction every day for 4 consecutive weeks. Gross anti-fatigue effects of HW were assessed by swimming endurance capacity, metabolic activities, and immune-redox activities. Metabolic activities such as blood glucose, lactate, glycogen, and lactate dehydrogenase (LDH) as well as immune-redox activities such as reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxidase (GPx), catalase, and the related cytokines were evaluated to elucidate underlying mechanism. HW group showed a higher swimming endurance capacity (p<0.001) than NC and PW groups. Positive metabolic effects in HW group were revealed by the significant reduction of blood glucose, lactate, and BUN after 4 weeks (p<0.01), as well as the significant increase of liver glycogen (p<0.001) and serum LDH (p<0.05). In parallel, redox balance was represented by lower ROS, NO (p<0.01), and increased level of GPx (p<0.05) and liver catalase (p<0.05) than PW group. In line, the decreased levels of TNF-α (p<0.01), IL-6 (p<0.001), and IL-23 (p<0.05) in HW group revealed positive cytokine profile compared to PW. This study shows anti-fatigue effects of hydrogen water (HW) drinking in chronic forced swimming mice via metabolic coordination and immune-redox balance. In that context, HW could be applied to the alternative and safety fluid remedy for chronic fatigue control.