earticle

영어

Non-thermal plasma water has dual function of anti-oxidant effect and anti-microbial effect, so it is useful apply several disease including atopic dermatitis and athlete foot. Atopic dermatitis (AD) is a chronic inflammatory skin disease described not only by the skin-barrier impairment and dysfunctional immune balance but also by increased oxidative stress. The infiltration of immune cells releases bioactive substances including reactive oxygen species. Here, we introduced a potential agent to manage AD in the form of non-thermal plasma water (NTPW) generated by electrical discharger (Plus Co., Ltd.) in water on the regulation of immune markers and antioxidant markers in dinitrochlorobenzene-induced hairless mice. Recent studies found its promising ability of activating, controlling and catalyzing reactions and complex biochemical procedures. NTP brings a big potential in wound healing, such as efficient disinfection or sterilization, therapy of various skin infections or tissue regeneration. In us in vivo experiment, atopic dermatitis was induced by application of 1% DNCB for the first week and alternate 0.5% DNCB application for the following 4-week NTPW treatment. While the regulatory immune and oxidative stress effects of NTP water was found to be more improved than no treatment group which is comparable to the positive controls. NTP water reduced the severity of dermatitis, including erythema/hemorrhage, edema, erosion and scaling, and skin scratching, total WBC specifically the neutrophils and lymphocytes; IgE level is also slightly decreased. ROS and NO in skin lysate showed an improved normalized level comparable to normal control. Accordingly, the antioxidant enzymes including glutathione peroxidase, superoxide dismutase, catalase, and MDA normalized the oxidative stress levels in skin lysate; these could play role in prohibiting AD aggravation. Furthermore, our results suggest that the NTP mechanism of action in AD is regarded with the regulation of immune response in acute phase in AD, and also to the inhibition of neutrophils, lymphocyte, and Th2-mediated IgE overproduction, marking an anti-inflammatory effect. Recent studies depict the association of atopic dermatitis with excessive skin floral invasion especially Staphylococcus aureus. Here, we introduced a possible agent to manage AD in the form of non-thermal plasma (NTP), a partially ionized water where energy is stored in the electron without any change of temperature. Recent studies found its promising effect in different biological application because of its enriched properties of H2O2, OH-, HOCl, O3, and other ions. In this randomized, double blind controlled study, 7 children aged 3 to 17 years old undergone a two-week NTP water bathing intervention in Yeongwol, Republic of Korea. Water samples were obtained aseptically before and after of bathing in NTPW to check the colony forming unit of S. aureus. Previously, in vitro results showed that has remarkable antibacterial and antifungal effect in TW. Before and after skin conditions were also compared by an automated dermatological scale. Our cumulative results showed reduction of Staphylococcus aureus count from patient’s skin swab. In addition, NTPW revealed slightly improved skin condition including moisture, elasticity, roughness and wrinkle. These results could open new horizons in medical dermatology as a management and treatment scale of AD. Overall, our findings strongly recommend that spraying and bathing with NTPW could be a promising therapeutic approach in ROS related disease and skin infectious disease.