Antioxidant Effect of Natural Fullerene (Shungite) in H2O2-Induced Oxidative Stress in vitro


Ma. Easter Joy Sajo, Cheol-Su Kim, Kim Soo-Ki, Kyu-Jae Lee

피인용수 : 0(자료제공 : 네이버학술정보)



As fullerene-based compound application rapidly increased in health industry, the need of toxicological and biological effects is in demand. Here, we investigate the potential antioxidant properties of mineral-rich shungite and less mineral shungite in olive oil, fullerene-containing compound, against H2O2-induced oxidative stress in HaCaT cells. The composition of the less mineral shFig. 5. Inflammatory cytokines mediate H2O2-Induced Oxidative Stress. The concentration of (A) TNF-α, (B) IL-1β, (C) IL-10 cytokine was determined using ELISA after pure fullerene, mineral-rich shungite, and mineral-less shungite treatment.ungite is 94.79% atomic carbon analyzed by EDX X-ray spectrophotometer. Cell viability was significantly decreased by the exposure of mineral-rich shungite, mineral-less shungite, and pure fullerene C60 (25, 50, 100, and 200 μg/mL) for 24 hrs in the cultured HaCaT cells. Mineral-rich and less-mineral shungite compared to pure fullerene did not induce toxicity in vitro, therefore natural mineral shungite is safer to use for further investigation of its antioxidant property. Intracellular reduced glutathione (GSH) and catalase level were also decreased compared to the level of the non-treated control group during the exposure period, while the level of super oxide dismutase (SOD) did not have significant change. Intracellular cytokine levels of IL-10, IL-1β and TNF-α were increased suggesting an induced inflammatory response by shungite. This study suggests that natural mineral shungite could be a novel antioxidant and could provide a new insight for oxidative and inflammatory related disease treatment.


 Materials and Methods
  1. Cell culture
  2. Preparation of pure fullerene, mineral- rich shungite, mineral-less shungite
  3. Induction of oxidative stress
  4. Cell Viability
  5. Determination of ROS Generation
  6. Endogenous Enzyme Activity: Glutathione Peroxidase Activity
  7. Superoxide dismutase (SOD) Assay
  8. Catalase Assay
  9. Enzyme-Linked Immunosorbent Assay(ELISA)
  10. Data management and Analysis.
  1. Effect of shungite in HaCaT cell viability
  2. Intracellular ROS mediates H2O2-induced oxidative stress in shungite-treated cells
  3. Endogenous enzyme activities mediates H2O2-induced oxidative stress
  4. Inflammatory cytokines mediate H2O2-induced oxidative stress.


  • Ma. Easter Joy Sajo Department of Environmental Medical Biology
  • Cheol-Su Kim Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 26426, Republic of Korea
  • Kim Soo-Ki Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 26426, Republic of Korea
  • Kyu-Jae Lee Department of Environmental Medical Biology, Institute for Poverty Alleviation and International Development, Yonsei University, Wonju, Gangwon 26493, Republic of Korea


자료제공 : 네이버학술정보

    함께 이용한 논문

      ※ 기관로그인 시 무료 이용이 가능합니다.

      • 4,000원

      0개의 논문이 장바구니에 담겼습니다.