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As fullerene-based compound application rapidly increased in health industry, the need of toxicological and biological effects is in demand. Here, we investigate the potential antioxidant properties of mineral-rich shungite and less mineral shungite in olive oil, fullerene-containing compound, against H2O2-induced oxidative stress in HaCaT cells. The composition of the less mineral shFig. 5. Inflammatory cytokines mediate H2O2-Induced Oxidative Stress. The concentration of (A) TNF-α, (B) IL-1β, (C) IL-10 cytokine was determined using ELISA after pure fullerene, mineral-rich shungite, and mineral-less shungite treatment.ungite is 94.79% atomic carbon analyzed by EDX X-ray spectrophotometer. Cell viability was significantly decreased by the exposure of mineral-rich shungite, mineral-less shungite, and pure fullerene C60 (25, 50, 100, and 200 μg/mL) for 24 hrs in the cultured HaCaT cells. Mineral-rich and less-mineral shungite compared to pure fullerene did not induce toxicity in vitro, therefore natural mineral shungite is safer to use for further investigation of its antioxidant property. Intracellular reduced glutathione (GSH) and catalase level were also decreased compared to the level of the non-treated control group during the exposure period, while the level of super oxide dismutase (SOD) did not have significant change. Intracellular cytokine levels of IL-10, IL-1β and TNF-α were increased suggesting an induced inflammatory response by shungite. This study suggests that natural mineral shungite could be a novel antioxidant and could provide a new insight for oxidative and inflammatory related disease treatment.