Alkaline-reduced water (ARW) was first developed in Japan, and its effectiveness has been explored in different health related fields. Generally, ARW is generated by electrolysis, whereby water (H2O) decomposes into oxygen (O2) and hydrogen gas (H2) due to an electric current. ARW also can be generated with additives, such as calcium or magnesium. ARW is characterized as having high pH, supersaturated hydrogen, and negative oxidation -reduction potential (ORP). Hydrogen-rich water has been introduced as a feasible therapeutic strategy for health promotion and disease prevention. Studies have shown that ARW has antioxidant properties and thus helping counteract free radicals in the body. These free radicals can cause premature aging and other conditions. The chemical properties of ARW, specifically its pH, are believed to contribute to its beneficial effects. Here, we hypothesized that the simple method of boiling water could increase the alkalinity and reduction power of water. The impact of simply boiling water, which is not only to increase its alkalinity and reducing the ORP, but also allows it to reduce the reactive oxygen species (ROS) levels in the body, raises the long-term possibility that drinking boiled water might prevent oxidative stress-related diseases.
Materials and Methods
1. pH and ORP measurement
2. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay