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Purpose: The study aimed to identify factors influencing work-life balance in female service workers. Methods: A cross-sectional descriptive study was conducted with an online survey of 234 service workers using volunteer panels. Data were collected using a self-administered questionnaire from July 16 to 24, 2017. Major variables included work-life balance, resilience, emotional intelligence, job stress, and fatigue. Using the SPSS 24.0 program, descriptive statistics, t-test, ANOVA, correlation analysis, and multiple regression analysis were conducted. Results: The mean score of the work-life balance level of female service workers was 2.9±0.85 on a total score of 6. A multiple regression analysis revealed that smoking, resilience, job stress and fatigue were statistically significant factors in the model (p<.001), with an explanatory power of 50.3%. Emotional intelligence was not significantly associated with work-life balance. Conclusion: The level of work-life balance was considerably low in female service workers. This study suggests that nursing interventions to enhance work-life balance are required at both the individual and organizational levels.