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The purpose of this study was to develop Smoking Prevention Empowerment Scale for Adolescents (SPES-A) based on a conceptual framework of psychological empowerment theory and evaluate its validity and reliability. The development process of initial items involved literature review, interviewing adolescents, and expert survey. Subsequently, a content validity evaluation and pilot survey were conducted. Participants were 594 adolescents (17 excluded) aged 10-18 years recruited to test reliability and validity of the preliminary scale. The final version of the SPES-A was a 4-point Likert type scale, consisting of 15 items in 4 categorized factors, including: “competence” (3 items), “meaningfulness” (4 items), “self-determination” (4 items), and “impact” (4 items). The Cronbach’s α coefficient was .89. Construct validity of SPES-A was verified using a confirmatory factor analysis. Factor loadings of the 15 questions ranged from .51 to .83. Criterion validity showed significant correlations (r=-.54, .54, p<.001). The psychometric evaluation of the SPES-A demonstrated an acceptable validity and reliability. However, further study is needed to retest the verification of the factor analysis related to factor 2 (meaningfulness) and factor 3 (self-determination). The SPES-A can be used as a tool to test the effect of smoking prevention programs for adolescents.