STR and SNP have been widely used genetic markers to confirm family relations. STR has been deemed very useful marker to confirm human identif ication and relations due to that everyone has different short tandem repeats (STRs) which is inhe rited indepe ndently. Des pite of weaker power of locus discrimination compared with STR, SNP features lower mutation rate (10·8) and binary polymorphism, which facilitate confirmation of family relations. In addition, applying next generation sequencing is now able to experiment larger number of SNP markers. In this study to determine relations of sibling, 17 STR and 169 SNP markers were used to see whether these markers have confidence to be used in the fi eld. 17 STR markers were detected in the full-sibling analysis and 140 SNP markers were analyzed among 169 SNP markers. When likelihood ratio value is more than 1, all true positive were detected (43 pairs), false negative were not detected and faJse positive pairs were more detected STR (47 pairs) than SNP (S pairs). Therefore FuJJ-sibJing anaJysis was efficient to use with the STR and SNP.
II. 실험 방법
2.2 STR 분석을 위한 증폭 및 전기영동
2.3 마커 정보 및 Multiplex PCR, DNA Chip 분석
2.4 형제지수 분석