원문정보
초록
영어
Background: The use of the extracts from evening primrose seeds as a health functional food has been gradually increased. Therefore, the monitoring and screening process has been considerably required for its quality control. Objective: This study aimed to estimate the measurement uncertainty associated with determination of penta-o-galloyl β-D-glucose (PGG) in extracts from evening primrose seeds by high-performance liquid chromatography. Methods: The sources of measurement uncertainty was expressed in accordance with mathematical/statistical theories of GUM & EURACHEM Guide. The expanded uncertainty was calculated by using the relative standard uncertainty between analytical result and sources of uncertainty in measurement (sample weight, final volume, extraction volume, standard solution, matrix and instrument etc). Results: In the results of 95% confidence interval, the uncertainty in measurement was 10,253.34 ± 1,844.50 μg/kg (k = 2.0). Conclusion: In this study, it showed that the value of uncertainty in measurement for determination of PGG in extracts from evening primrose seeds by HPLC has about 18.0% influence on PGG contents of the analytical results. The results would be very useful for the monitoring and screening of evening primrose seeds marketed in Korea for its quality control as dietary supplement.
목차
연구 방법
실험재료
표준용액의 조제
시험용액의 조제
기기분석 조건
불확도 추정방법
모델관계식 설정
요인별 측정값과 표준불확도
검량곡선의 불확도
합성표준불확도
확장불확도
연구 결과
시험용액 조제 시 불확도
표준용액의 순도 및 분자량의 불확도
표준용액 제조과정의 불확도
검량곡선의 불확도
시료의 반복측정에 따른 불확도
회수율 및 정밀성의 불확도
합성표준불확도 및 확장불확도
불확도 요인의 상대기여도
고찰 및 결론
참고문헌