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양송이 품종과 수집 균주간의 Inter-simple sequence repeat (ISSR) 마커 분석

원문정보

Analysis of Inter-simple sequence repeat (ISSR) markers in cultivars and collected strains of button mushroom (Agaricus bisporus)

남윤걸, 공원식, 장갑열, 신평균, 오민지, 임지훈, 구창덕, 오연이

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초록

영어

A. bisporus is the fifth most cultivated mushroom in Korea, and approximately 10,757 tons were cultivated in 2015. The genetic diversity of collected strains in Korea and commercial cultivars was analyzed using inter-simple sequence repeat (ISSR) markers. ISSR markers known to be comparable among A. bisporus spp. were selected from various markers. Totally, 16 markers, namely the ISSR markers 807, 808, 810, 811, 834, 835, 836, 841, 842, P3, P8, P17, P22, P30, P38, and P39, were evaluated to discriminate between ASI 1110, 1114, 1115, 1238, 1246, 1365, 1366, and 1369 for selecting suitable markers in 16 markers. The ISSR markers P31, P38 and P39 exhibited various fingerprints that could help classify the strains in species. Using the three markers, genetic relationships among 39 strains, including commercial cultivars, such as SaeA and SaeYeon, were analyzed using the UPGMA method. The results of the analysis of the genetic relationships between commercial cultivars and collected strains in Korea confirmed that the commercial cultivars were different from the collected strains in Korea. These results suggested that the ISSR markers P31, P38, and P30 could be used for selecting the commercial cultivars of A. bisporus.

목차

ABSTRACT
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저자정보

  • 남윤걸 Youn-keol Nam. 농촌진흥청 국립원예특작과학원 버섯과, 충북대학교 산림학과
  • 공원식 Won-Sik Kong. 농촌진흥청 국립원예특작과학원 버섯과
  • 장갑열 Kab-Yeul Jang. 농촌진흥청 국립원예특작과학원 버섯과
  • 신평균 Pyung-Gyun Shin. 농촌진흥청 국립원예특작과학원 버섯과
  • 오민지 MinJi Oh. 농촌진흥청 국립원예특작과학원 버섯과
  • 임지훈 Ji-Hoon Im. 농촌진흥청 국립원예특작과학원 버섯과
  • 구창덕 Chang-Duck Koo. 충북대학교 산림학과
  • 오연이 Youn-Lee Oh. 농촌진흥청 국립원예특작과학원 버섯과, 충북대학교 산림학과

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