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Purpose: The purpose of the study was to investigate the effects of subtraction meditation on perceived stress, self-efficacy, blood pressure, and pulse rate in paramedic students. Methods: A quasi-experimental study with non-equivalent comparison groups using pre and post-test design was performed. The experimental group consisted of 27 participants who attended the meditation program, and the control group consisted of 26 non-participants of the program. The data were analyzed using SPSS/WIN 24.0. Results: There were a statistically significant differences between the experimental group and the control group in perceived stress(t=2.301, p=.026), systolic pressure(F=18.531, p=.000), diastolic pressure(F=9.745, p=.003), and pulse rate(F=33.869, p=.000) between pretest and posttest results. Conclusion: The subtraction meditation program can help reduce perceived stress, systolic pressure, diastolic pressure, and pulse rate.