earticle

영어

Purpose: 6-Shogaol is one of the spicy flavor ingredients of ginger and hydrolysis product of gingerols. The purpose of this study is to investigate the potential of 6-shogaol as a natural cosmetic raw material by examining cell protection and inflammation inhibitory activity in human dermal fibroblasts (HDFs). Methods: To verify the cytoprotective effects of 6-shogaol, cell viability was measured by water-soluble tetrazolium salt (WST-1) assay. To verify the anti-inflammatory effects of 6-shogaol, we performed nuclear factor kappa-light-chain-enhancer of activated B cells (NFΚB) promoter luciferase assay to check the expression of NFΚ B. Additionally, the expression of inflammation related gene such as cyclooxygenase 2 (COX2), receptor for advanced glycation end product (RAGE), tumor necrosis factor alpha (TNFΑ), interleukin 6 (IL6), and interleukin 8 (IL8) mRNA was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Results: 6-Shogaol showed no toxicity in HDFs at concentrations of 5, 10, 20, and 40 μM, respectively and the cell viability was increased in a dose dependent manner. qRT-PCR analysis showed 6- shogaol treatment downregulated the expression of NFΚB, COX2, RAGE, TNFΑ, IL6, and IL8 in a dose dependent manner, resulting that 6-shogaol leads to protective activities against inflammatory responses in HDFs. Conclusion: As mentioned above, 6-shogaol restored cell viability and decreased the expression of inflammatory factors in a dose dependent manner. Consequently, these results suggest the possibility of 6-shogaol as cosmetic material preventing skin aging, through identified functions on cytoprotection and anti-inflammation.

한국어

목적: 6-Shogaol은 생강(Zingiber officinale)의 매운맛을 내는 성분인 gingerols의 가수분해 산물로서, 본 연구에서는 6-shogaol 의 인간진피섬유아세포에서의 세포보호와 염증억제 효능을 검증함으로써 천연유래 화장품 원료로서의 사용가능성을 알아보고 자 한다. 방법: 6-Shogaol의 세포보호 효과를 검증하기 위하여, water-soluble tetrazolium salt (WST-1) assay를 이용하여 세포 생존율을 측정하였다. 6-Shogaol의 항염증 효과를 검증하기 위하여, nuclear factor kappa-light-chain-enhancer of activated B cells (NFΚB) promoter luciferase assay를 이용하여 염증반응 전사인자인 NFΚB의 발현을 측정하였으며 염증관련 유전자 들인 cyclooxygenase 2 (COX2), receptor for advanced glycation end product (RAGE), tumor necrosis factor alpha (TNFΑ), interleukin 6 (IL6), interleukin 8 (IL8) 발현량을 quantitative real-time polymerase chain reaction (qRT-PCR)를 이용하여 측정 하였다. 결과: 6-Shogaol은 5, 10, 20, 40 μM 각각의 농도에서 인간진피섬유아세포에 대한 독성을 보이지 않았으며 농도의존적 으로 10 J/cm²의 ultraviolet A (UVA)에 의해 손상된 인간진피섬유아세포의 생존율을 회복시켰다. 대표적인 염증반응 전사인자인 NFΚB의 promoter 활성이 6-shogaol에 의해 농도의존적으로 감소되었으며, 염증유발 유전자인 COX2, RAGE mRNA와 각종 염 증관련 사이토카인인 TNFΑ, IL6, IL8 mRNA의 발현도 6-shogaol에 의해 농도의존적으로 감소되었다. 결론: 6-Shogaol은 세포 보호 및 항염증에 대한 확인된 효능을 통해 피부노화를 예방하는 화장품 원료로서의 가능성을 보여줄 수 있으리라 사된료다.

중국어

目的: 6-Shogaol是生姜 (Zingiber officinale ) 的辛辣成分gingerols的水解产物。探讨6-shogaol对人皮肤成纤维细胞 的细胞保护和抗炎功效，从而鉴定6-shogaol作为天然化妆品原料的应用可行性。方法: 为确认6-Shogaol的细胞保 护效果，采用water-soluble tetrazolium salt (WST-1) 法测定细胞生存率实验。为验证6-Shogaol的抗炎功效，采用 nuclear factor kappa-light-chain-enhancer of activated B cells (NFΚB ) promoter luciferase assay测定炎症反应转 录因子NFΚB 的表达，以及采用 quantitative real-time polymerase chain reaction (qRT-PCR) 法确定炎症相关遗传因 子 cyclooxygenase 2 (COX2 )，receptor for advanced glycation end product (RAGE )，tumor necrosis factor alpha (TNFΑ )，interleukin 6 (IL6 )，interleukin 8 (IL8 ) 的表达量。结果: 6-Shogaol在5、10、20、40 μM的浓度下，对人皮 肤成纤维细胞没有毒性。6-Shogaol对被10 J/cm² ultraviolet A (UVA) 损伤的人皮肤成纤维细胞的生存率呈浓度依赖性 恢复。代表性的炎症转录因子NFΚB 的promoter活性被6-shogaol呈浓度依赖性降低。炎症诱发遗传因子COX2 ，RAGE mRNA和各种炎症相关细胞因子TNFΑ 、IL6 、IL8 mRNA的表达被6-shogaol呈浓度依赖性降低。结论: 通过以上研究确 认了6-Shogaol具有细胞保护和抗炎功效，因此作为预防抗衰老的化妆品原料充分具有可行性。