earticle

영어

Purpose: We investigated the applicability of extracts from Oenanthe javanica (OJ), Perilla frutescens (PF), and Zanthoxylum piperitum (ZP) as cosmetic ingredients by confirming their anti-oxidant and anti-inflammatory effects in vitro. Methods: Essential oils were obtained from OJ, PF, and ZP by simultaneous steam distillation–extraction, and their volatile components were analyzed by gas chromatography-mass spectrometry (GC-MS). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities of these essential oils were then investigated to assess their anti-oxidant effects, and 5-(3-caroboxymeth-oxyphenyl)-2H-tetrazolium inner salt (MTS) and nitric oxide (NO) inhibition assays were performed to measure their anti-inflammatory effects. Results: We identified 15, 9, and 18 volatile compounds in the essential oils obtained from OJ, PF, and ZP, respectively. The principal volatile compounds were β-caryophyllene (20.46%) in OJ, dipentene (21.55%) in ZP, and α-pyrone (58.14%) in PF. We also found that the anti-oxidant effects of the essential oils were greatest for OJ (91%), intermediate for PF (72%), and lowest for ZP (26%) and was concentration-dependent for all three. Furthermore, the cell viability of RAW 264.7 cells under lipopolysaccharide (LPS)-induced oxidative stress when treated with a 10% concentration of the essential oils was highest for ZP (107.7%), intermediate for PF (95.3%), and lowest for OJ (94.8%). The anti-inflammatory effects in mouse macrophages with LPS-induced inflammation were also greatest for ZP (71% NO inhibition), intermediate for PF (43% NO inhibition), and lowest for OJ (22% NO inhibition). Conclusion: Essential oils from OJ, PF, and ZP can be used as natural cosmetic ingredients with anti-oxidant and anti-inflammatory activities to prevent skin inflammation in humans.

한국어

목적: 미나리, 깻잎, 초피 정유의 항산화와 항염 효과를 in vitro에서 조사하여 화장품 소재로서의 활용 가능성을 확인하고자 하였 다. 방법: 수증기 증류법에 의한 미나리, 깻잎, 초피 정유의 gas chromatography-mass spectrometry (GC-MS) 향기성분 분석 을 진행하였고, 항산화 활성을 확인하기 위해 정유의 2,2-diphenyl-1-picrylhydrazyl (DPPH) 라디칼 소거능을 조사하였다. 또한 정유의 항염 활성을 확인하기 위해 5-(3-caroboxymeth-oxyphenyl)-2H-tetrazolium inner salt (MTS) assay, nitric oxide (NO) assay를 실시하였다. 결과: GC-MS실험결과, 미나리, 깻잎, 초피 정유의 향기성분이 15, 9, 18개로 측정되었다. 미나리 및 초피 정 유에서는 각각 항염 효과를 나타내는 β-caryophyllene (20.46%), dipentene (21.55%) 성분을 주로 포함하고 있으며, 깻잎에서는 주 요 향료로 사용되는 α-pyrone (58.14%)이 대표적인 성분으로 확인되었다. DPPH 라디칼에서는 미나리(91%)>깻잎(72%)>초피(26%) 정유 순으로 농도의존적으로 라디칼이 소거되었음을 확인하였다. 세포생존은 RAW 264.7 세포에 10% 농도의 미나리, 깻잎, 초피 정유와 lipopolysaccharide (LPS)를 처리하였을 때, 94.8%, 95.3%, 107.7%로 세포생존율이 나타나 10% 농도까지 독성이 없는 것으 로 측정되었다. 또한 10% 농도의 미나리, 깻잎, 초피 정유에 의해 각각 22%, 43%, 71%의 nitric oxide (NO) 생성 억제 효과를 나타 내었다. 특히 초피 정유에서 높은 NO 생성 억제력이 나타났다. 결론: 미나리, 깻잎, 초피 정유는 현대인들의 피부염증 예방에 도움 이 되는 항산화, 항염 천연 화장품 성분으로 이용될 수 있으리라 사료된다.

중국어

目的: 在in vitro中，分别调查水芹、紫苏叶、花椒等精油的抗氧化和抗炎功效，鉴定作为化妆品原料的可行性。方法: 采 用水蒸气蒸馏法分别提取水芹、紫苏叶、花椒的精油，并用气相色谱-质谱仪 (gas chromatography-mass spectrometry; GC-MS)分析芳香成分。采用2,2-diphenyl-1-picrylhydrazyl (DPPH)自由基消除能力来评估精油的抗氧化活性。采用 5-(3-caroboxymeth-oxyphenyl)-2H-tetrazolium inner salt (MTS) assay和nitric oxide (NO) assay来评估精油的抗炎活性。 结果: GC-MS实验结果，水芹、紫苏叶、花椒的精油中，分别得到15、9、18个挥发性化合物。在水芹和花椒精油中， 分别检测到具有抗炎效果的β-caryophyllene (20.46%)，dipentene (21.55%)；在紫苏叶精油中，检测到主要香料成分 α-pyrone (58.14%)，α-pyrone在紫苏叶精油中是主要代表性成分。按水芹 (91%)、紫苏叶 (72%)、花椒 (26%)的顺序检 测到浓度依赖性具有DPPH自由基消除能力。在RAW 264.7细胞中，分别用10%浓度的水芹、紫苏叶、花椒等的精油和 lipopolysaccharide (LPS)处理时，细胞生存率显示为94.8% (水芹)、95.3% (紫苏叶)、107.7% (花椒)。因此，各精油的浓 度在10%以下时，显示出没有毒性。在nitric oxide (NO)生成抑制效果结果中，10%浓度的水芹、紫苏叶、花椒精油分别 显示22%、43%、71%的抑制效果。特别是花椒精油对NO的生成具有很强的抑制作用。结论: 水芹、紫苏叶、花椒的精 油可以作为抗氧化和抗炎症的天然化妆品原料，有助于预防现代人的皮肤炎症。