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RESEARCH ARTICLE

Efficacy of a Cosmetic Material from Complex Extracts of Vaccinium spp., Phellinus linteus, Castanea crenata, and Cimicifuga heracleifolia

원문정보

블루베리, 상황, 율피, 승마 복합추출물의 화장품 소재로서의 효능

Young-Ah Jang

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초록

영어

Purpose: To confirm the possibility of using complex extracts of Vaccinium spp., Phellinus linteus, Castanea crenata, and Cimicifuga heracleifolia for cosmetic substances, we tested anti-oxidant and anti-inflammatory activities. Methods: The complex extracts of Vaccinium spp., Phellinus linteus, Castanea crenata, and Cimicifuga heracleifolia were used in a ratio of 1:1:1:1, and these complexes were extracted in 70% ethanol. To evaluate the anti-oxidant activity, the complexes were concentrated and the scavenging activities of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) cation were assessed. The anti-inflammatory activity was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, western blot analysis, and real-time polymerase chain reaction (real-time PCR). Results: Complex extracts showed 83.1% DPPH and 98.4% ABTS+ radical scavenging activities at a concentration of 1000 μg/mL. The MTT assay revealed cell viability over 81.0% at 10, 25, and 50 μg/mL concentrations of the complex extracts and cell death over 21.0% at 100 and 250 μg/mL concentrations of the complex extracts. Therefore, the experimental concentration of the complex extracts was 10–50 μg/mL. Western blot analysis revealed that at 50 μg/mL concentration of the complex extracts, the expression level of nitric oxide (NO) was decreased by 28.0% and those of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2) were decreased by 32.8% and 48.0%, respectively. Real-time PCR showed a decrease in iNOS and COX2 mRNA expression levels in a concentration-dependent manner. Conclusion: Complex extracts have excellent anti-oxidant and anti-inflammatory effects, and we expect that these extracts will serve as functional cosmeceutical materials.

한국어

목적: 본 연구에서는 블루베리, 상황, 율피, 승마 복합추출물의 화장품 소재로서의 가능성을 확인하기 위해 항산화와 항염증 활 성을 측정하였다. 방법: 블루베리, 상황, 율피, 승마는 70% 에탄올에서 추출하였고, 복합추출물은 각 시료를 1:1:1:1의 비율로 사용하였다. 항산화 평가를 위해 복합물을 농도에 따라 처리하여 1,1-diphenyl-2-picrylhydrazyl (DPPH) 라디칼 소거능과 2,2’ -azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) 양이온 라디칼 소거능을 확인하였다. 항염증 활성평가는 과학적 으로 증명된 방법인 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 분석법, western blot 분석법, realtime polymerase chain reaction (real-time PCR) 분석법으로 측정하였다. 결과: 복합추출물은 1000 μg/mL 농도에서 DPPH 라 디칼 소거능 활성이 83.1%로 나타났으며 ABTS+ 라디칼 소거능 활성이 98.4%로 나타났다. MTT 분석법을 통한 세포 생존율은 10, 25, 50 μg/mL 농도에서는 생존율이 81.0% 이상으로 나타났고, 100, 250 μg/mL 농도에서는 세포사멸이 21.0% 이상으로 나타났 다. 따라서 복합물의 최종농도를 10–50 μg/mL로 사용하였다. Nitric oxide (NO) 생성은 50 μg/mL 농도에서 28.0% 감소되었고, western blot 분석을 통한 inducible nitric oxide synthase (iNOS)와 cyclooxygenase 2 (COX2) 단백질 발현은 50 μg/mL 농도에서 각 32.8%, 48.0%의 감소를 나타내었다. Real-time PCR 결과 iNOS와 COX2의 mRNA 발현율은 농도 의존적으로 감소되었다. 결론: 복합추출물은 항산화 및 항염증에 탁월한 효능이 있으며 이러한 추출물이 향후 약용화장품 소재로서 사용되기를 기대한다.

중국어

目的: 探讨蓝莓、桑黄、栗皮、升麻复合提取物作为化妆品原料的可行性,测定其抗氧化和抗炎活性。方法: 在70%乙 醇中,分别提取蓝莓、桑黄、栗皮、升麻的提取物,将这些提取物按1:1:1:1的比例混合作为复合提取物。为了评价复合 提取物的抗氧化活性检测1,1-diphenyl-2-picrylhydrazyl (DPPH) 自由基消除能力和2,2’-azino-bis (3-ethylbenzothiazoline- 6-sulphonic acid) (ABTS) 阳离子消除能力。抗炎症活性通过科学证明的方法,即,3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) 分析法、western blot法、real-time polymerase chain reaction (real-time PCR) 等方 法来评价。结果: 1000 μg/mL的复合提取物的DPPH自由基消除能力为83.1%,ABTS+自由基消除能力为98.4%。细胞生存 率通过MTT分析法来测定,在10, 25, 50 μg/mL的浓度下,细胞生存率显示出81.0%以上,在100, 250 μg/mL的浓度下, 细胞死亡达到21.0%以上。因此,复合提取物的最终使用浓度为10–50 μg/mL。在50 μg/mL浓度下,nitric oxide (NO) 的 生成减少了28.0%。通过western blot法检测inducible nitric oxide synthase (iNOS) 和cyclooxygenase 2 (COX2) 的蛋白质 表达,其结果显示:复合提取物在50 μg/mL浓度下,iNOS和COX2分别减少了32.8%,48.0%。Real-time PCR结果显示: iNOS 和COX2 的mRNA表达水平以浓度依赖性降低。结论: 复合提取物具有卓越的抗氧化和抗炎症功效,期待这些提取物 将成为药妆品使用。

목차

Abstract
 Introduction
 Methods
  1. 추출물의 제조
  2. DPPH 라디칼 소거능 측정
  3. ABTS+ 라디칼 소거능 측정
  4. 세포독성 측정
  5. NO 생성 저해능 측정
  6. Western blot
  7. Real-time PCR
  8. 통계처리
 Results and Discussion
  1. DPPH 라디칼 소거활성
  2. ABTS+ 라디칼 소거활성
  3. 세포생존율
  4. NO 생성 저해활성
  5. iNOS, COX2 단백질 발현 저해활성
  6. iNOS , COX2 의 mRNA 발현측정
 Conclusion
 Acknowledgements
 References
 국문초록
 中文摘要

저자정보

  • Young-Ah Jang 장영아. Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan-si, Gyeongsangbuk-do, Korea

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