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Objectives: The purpose of this study was to investigate the effect of Sexual Knowledge and Sexual Attitude on the Sexual Coping Behavior among university students and presents basic data for helping healthy Sexual Culture. Methods: A survey was held to the university students in Seoul and Chungcheong cities from April 11 to April 25, 2016. of 237 copies were in the final analysis. For data analysis, SPSS 21.0 for Windows was used. Results: As a result, there were correlations between variables, Sexual Coping Behavior of University students were affected by the gender, distorted sex, have the experience of sexual intercourse and accepted sex. Conclusions: It is need to development program and counseling for establishment of the healthy sex culture of university students.