한우의 혈액시료 처리 방법(시간, 온도) 따른 혈액 내 사이토카인 및 생화학적 변화


Blood Sample Handling Method (Time, Temperature) for Cytokines and Biochemical Analysis in Hanwoo

김성범, 조아라, 최창용, 류재규, 도윤정

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Serum plays a role as biomarker in the assessment of health condition or severity of diseases. The separation method, storage temperature and duration affect the levels of cytokines and its biochemical constituents. In this study, blood sampling was performed from Hanwoo cattle through jugular venipuncture. All blood samples were kept at 26±3℃ for 1~2 h and then separated into four conditions (groups a-d). For group (a), the serum was immediately separated (control group) and stored at -70℃; for group (b) the separated serum was kept in ice box for 2 h and then stored at -70℃; for group (c) the collected blood samples were stored in ice box for 2hrs before serum separation; and for group (d); the whole blood was kept at 4℃ for 24hrs before serum separation and then stored at -70℃. All serum samples were stored at -70℃ until analysis. Depending on serum separation time, storage period and temperature, IFN-γ level revealed no significant differences; whereas, TNF-α level was significantly lower (p<0.05) in group (d) than in groups (a-c). Besides, concentration of albumin and total protein were significantly higher (p<0.01) in (d) than that in group (a). The levels of magnesium and calcium also echoed similar results (p<0.05). But G-GTP was significantly lower (p<0.05) in group (d) compared with other groups. Therefore, considering the distance between the farm and the laboratory, it is necessary to minimize the effect of temperature and storage time on serum cytokine and biochemical concentration.


 Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 공시 축 및 혈액채취
  2. 혈청분리
  3. 혈청분석
  4. 통계분석
 Ⅲ. 결과 및 고찰
  1. 혈청 내 사이토카인 농도변화
  2. 혈청 분리 방법에 따른 생화학 검사
 Ⅳ. 요약


  • 김성범 Seong Bum Kim. 농촌진흥청 국립축산과학원 가축질병방역팀
  • 조아라 Ara Cho. 농촌진흥청 국립축산과학원 가축질병방역팀
  • 최창용 Chang Yong Choe. 농촌진흥청 국립축산과학원 가축질병방역팀
  • 류재규 Jae Gyu Yoo. 농촌진흥청 국립축산과학원 가축질병방역팀
  • 도윤정 Yoon Jung Do. 농촌진흥청 국립축산과학원 가축질병방역팀


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