원문정보
초록
영어
Recently, DNA vaccination is attracting attentions as a new therapeutic method for lifestyle diseases and autoimmune diseases. However, its clinical applications are limited because a safe and efficient gene transfer method has not been established yet. In this study, a new method of gene transfer was proposed which utilizes the jet injection and the magnetic transfection. The jet injection is a method to inject medical liquid by momentary high pressure without needle. The injected liquid diffuses in the bio tissue and the endocytosis is considered to be improved by the diffusion. The magnetic transfection is a method to deliver the conjugates of plasmid DNA and magnetic particles to the desired site by external magnetic field. It is expected that jet injection of the conjugates causes slight membrane disruptions and the traction of the conjugates by magnetic field induces the efficient gene transfer. In conclusion, the possibility of improvement of the gene expression by the combination of jet injection and magnetic transfection was confirmed.
목차
1. INTRODUCTION
2. METHODS
2.1. Magnetic field sources and magnetic particles
2.2. Treatment of animals in experiment
2.3. Evaluation of the gene expression
2.4. Evaluation of immune response
3. RESULTS AND DISCUSSIONS
3.1 Particle size distribution of conjugate of magnetite nanoparticle and plasmid DNA
3.2. Gene expression administered by jet injection and magnetic transfection
3.3. Immune response in rat
4. CONCLUSION
REFERENCES