원문정보
초록
영어
The olfactory bulbectomy (OBX) is a well-known method inducing ani-mal model of depression. Depression is associated with dysfunction of serotonin (5-hydroxytryptamine, 5-HT) system. In the present study, an-tidepressive effect of treadmill exercise was investigated using olfacto-ry bulbectomized rats. After bilateral bulbectomy, the rats in the tread-mill exercise groups were subjected to run on a treadmill for 30 min once a day during 28 days. Increased immobility time and decreased fast time in the forced swim test were observed in the olfactory bulbec-tomized rats. Sucrose preference in the sucrose preference test was decreased and activity in the open field test was also increased in the olfactory bulbectomized rats. Treadmill exercise decreased immobility time and activity and increased fast time and sucrose preference in the olfactory bulbectomized rats. Expressions of 5-HT and tryptophan hy-droxylase (TPH) in the dorsal raphe of rats were suppressed by OBX and treadmill exercise increased the expressions of 5-HT and TPH in the olfactory bulbectomized rats. Serotonin receptor type 1A (5-HT1A) expression in the dorsal raphe was reduced by OBX and treadmill exer-cise increased 5-HT1A expression in the olfactory bulbectomized rats. In the present study, treadmill exercise ameliorated OBX-induced depres-sive symptoms. The antidepressive effect of treadmill exercise might be ascribed to the enhancement of serotonergic function through upregu-lation of 5-HT1A expression in the dorsal raphe.
목차
INTRODUCTION
MATERIALS AND METHODS
Animals
Bilateral OBX surgery
Treadmill exercise protocol
Forced swimming test
Sucrose preference test
Open field test
Tissue preparation
Immunohistochemistry for 5-HT and TPH expressions
Western blot analysis for TPH and 5-HT1A expressions
Statistical analysis
RESULTS
Effect of treadmill exercise on immobility and fasting time
Effect of treadmill exercise on sucrose preference
Effect of treadmill exercise on activity
Effect of treadmill exercise on 5-HT and TPH expressions in the dorsal raphe
Effect of treadmill exercise on TPH and 5-HT1A expressions in the dorsal raphe
DISCUSSION
CONFLICT OF INEREST
ACKNOWLEDGMENTS
REFERENCES