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Abstracts for poster Presentation

Analysis of fucosylation in liver-secreted N-glycoproteins from human hepatocellular carcinoma plasma using liquid chromatography with tandem mass spectrometry

초록

영어

Fucosylation of N-glycoproteins has been implicated in various diseases, such as hepatocellular carcinoma (HCC). However, few studies have performed site-specific analysis of fucosylation in liver-secreted proteins. In this study, we characterized the fucosylation patterns of liversecreted proteins in HCC plasma using a workflow to identify site-specific N-glycoproteins, where characteristic B- and/or Y-ion series with and without fucose in collision-induced dissociation were used in tandem mass spectrometry. In total, 71 fucosylated N-glycopeptides from 13 major liver-secreted proteins in human plasma were globally identified by LCMS/MS. Of the fucosylated N-glycopeptides, bi- and tri-antennary glycoforms were the most common ones identified in liver-secreted proteins from HCC plasma. Therefore, we suggest that this analytical method is effective for characterizing fucosylation in liver-secreted proteins.

저자정보

  • Eun Sun Ji Biomedical Omics Group, Korea Basic Science Institute, Ochang, Republic of Korea
  • Heeyoun Hwang Biomedical Omics Group, Korea Basic Science Institute, Ochang, Republic of Korea
  • Gun Wook Park Biomedical Omics Group, Korea Basic Science Institute, Ochang, Republic of Korea, Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Republic of Korea
  • Ju Yeon Lee Biomedical Omics Group, Korea Basic Science Institute, Ochang, Republic of Korea
  • Hyun Kyoung Lee Biomedical Omics Group, Korea Basic Science Institute, Ochang, Republic of Korea, Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Republic of Korea
  • Na Young Choi Biomedical Omics Group, Korea Basic Science Institute, Ochang, Republic of Korea, Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Republic of Korea
  • Hoi Keun Jeong Biomedical Omics Group, Korea Basic Science Institute, Ochang, Republic of Korea, Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Republic of Korea
  • Kwang Hoe Kim Biomedical Omics Group, Korea Basic Science Institute, Ochang, Republic of Korea, Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Republic of Korea
  • Jin Young Kim Biomedical Omics Group, Korea Basic Science Institute, Ochang, Republic of Korea
  • Jong Shin Yoo Biomedical Omics Group, Korea Basic Science Institute, Ochang, Republic of Korea, Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Republic of Korea

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