earticle

영어

Quercetin is suggested to be potentially beneficial for the prevention of various diseases. Enzymatically modified isoquercitrin was prepared by glycosylation of quercetin and the subsequent of oligoglucosylation of quercetin 3-O-β-glucoside (isoquercitrin) produced from quercetin. To enhance the production of isoquercitrin with high specificity, yield, and purity, an enzymatic bioconversion method was developed enzyme UGT78K1 from glycine max, which has quercetin transforming ability. UGT78K1 could effectively transform the quercetin into isoquercitrin. Enzymatically modified isoquercitrin was glucosylated to its oligoglucosyl quercetin 3-O-β-Glucoside by levansucrase of a genetically modified strain of Leuconostoc mesenteroides. levansucrase industrially important glycansucrase from L. mesenteroides.