earticle

영어

Gastric cancer has one of the highest cancer mortality rates worldwide, largely because of di fficulties in early-stage detection. Aberrant glycosylation in serum proteins is related with many human diseases including inflammation and various types of cancer. Aberrant glycosylation is desirable in order to improve the specificity and sensitivity for clinical use. Here, we combined protein-specific immunoaffinity purification, glycan release, and MS analysis to examine haptoglobin glycosylation of gastric cancer patients for glyco-markers. Interestingly, abundances of several tri- and tetra-antennary fucosylated N-glycans were increased in gastric cancer patients. Additionally, structural analysis via LC/MS/MS demonstrated that the fucosylated complex type N-glycans were mainly decorated with antenna fucose. In this study, we developed a targeted glycoproteomic approach using chip-based nano LC-QTOF MS and MS/MS following antibody-assisted targeted purification to discover glycan signatures of serum haptoglobin for gastric cancer. We could further obtain a specific structure of fucosylated molecules that are potential glyco-markers for gastric cancer via LC/MS/MS. The current study demonstrates that glycomic profiling of targeted serum haptoglobin via LC/MS and LC/MS/MS may be used as a powerful platform to monitor the specific glycosylation associated with gastric cancer.