earticle

영어

Capsosiphon fulvescens (commonly called Maesaengyi in Korea), a green algae, has been traditionally used in Korea for centuries as a functuinal food. Polysaccahrides (SPS-CF, 385 kDa) isolated from Capsosiphon fulvescens have been shown to exhibit various biological activities, including anti-coagulanting, immune modulating and anti-cancer properties. However, anti-osteoporosis activity of SPS-CF has been rarely explored. In this study, we investigated its anti-osteoporotic effects using RAW 264.7 osteoclastic cells. The results showed that SPS-CF have been dose-dependently reduced the TRAP activity in the RAW 264.7 cells, with an approximately 59 % reduction at 50 μg/ml, while SPS-CF did not reduced cell viability. The SPS-CF treatment reduced actin organization in RAW 264.7 cells and significantly decreased protein level of Carbonic anhydraseⅡ. The inhibition of osteoclast differentiation shown to be mediated by suppression of RANKL signaling pathway, as evidenced by decreased TRAF6, gelsolin and phosphorylation of Src. These results SPS-CF demonstrated for the first time that SPS-CF inhibits the osteoclast differentiation, suggestubg that SPS-CF can be a functional ingredient for health beneficial foods or therapeutic agents to treat osteoporosis.