Glycosylation reactions are widespread in nature, and involved almost all vital processes. Glycosylated compounds directly exert a wide range of functions, including energy storage, maintenance of the cellular integrity, information storage and transfer, molecular recognition, cell-cell interaction, cellular regulation, virulence and chemical defense. Glycosylated compounds are the most structurally diverse biomolecules, and their biosynthesis needs quite complex biological processes orchestrated by many enzyme systems. In Plants, various natural products are produced including diverse flavonoid derivatives. Mostly these metabolites are generally glycosides and are accumulated in the vacuole. Among C-glycosylflavones, comprising the various pharmacological activities, are biosynthesized from flavone via C-glycosylation of 2-hydroxyflavone or flavone. This is mediated by uridine diphosphate (UDP)-sugar dependent glycosyltransferase. The C-glycosyltransferase catalyzes the transfer of the glucose moiety to the aromatic carbon of the acceptor substrate. C-Glycosylflavones are involved in UV protection, defense against pathogens and inhibition of caterpillar growth. In this study, we tried to biosynthesize C-glycosylflavone in vivo and the product was confirmed by LC-MS.