Cell surface lectins in the immune system bind to glycans expressed on the surface of pathogens, the event that leads to stimulation of immune responses to pathogens. Mouse SIGN-R1 (SIGN-related 1) is known to recognize mannose-rich and fucose containing glycans in a Ca2+-dependent manner. When SIGN-R1 binds to glycans on host cells, bacteria or viruses, glycan antigens enter cells via lectin-mediated endocytosis to elicit immune activation. We applied carbohydrate microarrays containing various glycans to rapidly identify functional glycans that promote cell surface lectin-associated cellular responses. Because binding of glycan ligands to SIGN-R1 on the cell surface promotes production of reactive oxygen species (ROS), the functional glycans on the microarrays are readily identified by using a ROS fluorescent probe PF1. Glycan microarrays used in this study were fabricated by immobilizing xx (몇개) unmodified glycans on hydrazide-modified glass slides. SIGN-R1 expressing cells pre-treated with PF1 were applied to the glycan microarrays and the fluorescence intensity of the cells were then measured by using a confocal fluorescence microscopy or a microarray scanner. The results of microarray experiments revealed that several Man and Fuc bearing glycans stimulate ROS production, a phenomenon that was abrogated in the presence of a ROS scavenger or an NADPH oxidase inhibitor. The present study demonstrated at the first time that glycan microarrays serve as a useful tool to identify functional glycans that elicit cell surface lectin-associated cellular responses.