earticle

영어

Cell surface lectins in the immune system bind to glycans expressed on the surface of pathogens, the event that leads to stimulation of immune responses to pathogens. Mouse SIGN-R1 (SIGN-related 1) is known to recognize mannose-rich and fucose containing glycans in a Ca2+-dependent manner. When SIGN-R1 binds to glycans on host cells, bacteria or viruses, glycan antigens enter cells via lectin-mediated endocytosis to elicit immune activation. We applied carbohydrate microarrays containing various glycans to rapidly identify functional glycans that promote cell surface lectin-associated cellular responses. Because binding of glycan ligands to SIGN-R1 on the cell surface promotes production of reactive oxygen species (ROS), the functional glycans on the microarrays are readily identified by using a ROS fluorescent probe PF1. Glycan microarrays used in this study were fabricated by immobilizing xx (몇개) unmodified glycans on hydrazide-modified glass slides. SIGN-R1 expressing cells pre-treated with PF1 were applied to the glycan microarrays and the fluorescence intensity of the cells were then measured by using a confocal fluorescence microscopy or a microarray scanner. The results of microarray experiments revealed that several Man and Fuc bearing glycans stimulate ROS production, a phenomenon that was abrogated in the presence of a ROS scavenger or an NADPH oxidase inhibitor. The present study demonstrated at the first time that glycan microarrays serve as a useful tool to identify functional glycans that elicit cell surface lectin-associated cellular responses.