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학생구두발표

Development of DNA-based glycan microarray platform for on-chip enzymatic glycosylation

초록

영어

Synthesizing the specific glycan is important for analysis and understanding of interaction with glycan-binding proteins (GBP). Especially, on-chip enzymatic synthesis of glycan can be efficient and useful tool since this method can directly provide diverse glycans to use for analyzing carbohydrate-protein interaction on the chip. However, efficient identification of yield of the synthesized glycan on the chip remains a major challenge. In this work, we prepared novel glycan microarray platform in which glycan-conjugated single-stranded DNA was synthesized using DNA synthesizer, and the synthesized carbohydrate-oligonucleotide conjugates (COCs) were immobilized onto the complementary single-stranded DNA-modified glass slide. Due to the property of DNA to be possible for reversible hybridization and denaturation under certain condition, we effectively obtained the synthesized glycan on the chip, and optimized the synthesis condition for target glycans. In our study, we anticipate that this novel glycan microarray can be successfully used in a number of glycan-related studies and applications, including analysis of diverse glycan-protein interactions, characterization of glycan-processing enzymes, and high throughput detection of target pathogens for diagnosis.

저자정보

  • Hye Ryoung Heo Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea
  • Chang Sup Kim Department of Chemical Engineering, Pohang University of Science and Technology, School of Chemistry and Biochemistry, Yeungnam University, Gyeongsan 712-749, Korea
  • Jeong Hyun Seo Department of Chemical Engineering, Pohang University of Science and Technology, School of Chemical Engineering, Yeungnam University, Gyeongsan 712-749, Korea
  • Hyung Joon Cha Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea

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