원문정보
초록
영어
Recently, researches on plant polysaccharides have been increasingly important topics for the development of therapeutics for various diseases due to their multiple biological properties including anti-inflammation, wound healing, anti-hepatitis, anti-ulcer, and anti-neoplastic effects. The Camellia oleifera has been used extensively as cooking oil and traditionally applied as a medicine for stomachache and burning injury in China. The objectives of this study was to investigate the anti-osteoporotic effect and immunostimulating activity of Camellia oleifera polysaccharides (COP). Polysaccharides were isolated from the defatted C. oleifera seeds mainly by water extraction and 75% ethanol precipitation. Monosaccharide composition analysis by HPEAC-PAD showed that these polysaccharides consist of rhamnose, arabinose, galactose, glucose, and xylose as the major neutral sugars. COP was not toxic at up to 500 μg/ml in RAW 264.7 cells, and increased immunostimulating cytokines (TNF-α and IL-6) secretion in a dose-dependent manner. In addition, COP inhibited the receptor activator of NF-кB ligand (RANKL)-induced tartrate-resistance acid phosphatase (TRAP) activity and the formation of multinucleated osteoclasts. These results suggest that the COP may have potential anti-osteoporotic activity.