earticle

영어

Several novel approaches are underway for control of plant-parasitic nematode Meloidogyne incognita. This study was aimed to compare metabolome profiles of the nematode at all its developmental stages. Metabolome analysis was performed in 5 stages (egg, J2, J3, J4 and female) and peaks detected in CE-TOFMS. Among 209 putative metabolites detected (61 anionic mode and 148 cationic mode), 110 metabolites (56 anioic and 54 cationic modes) with available standards were quantified. Principal component analysis showed that the metabolites from stages female and J2; J3 and J4 stages grouped into clusters and metabolites of egg formed a unique cluster. Quantitative analysis indicated amino acids to be the dominant group of metabolites. Further, highly expressed metabolites included osmolytes proline, betaine, hydroxyproline; antioxidant molecules putrescine and glutathione; sensory regulators gama amino benzoic acid (GABA) and β alanine; organic acids lactic and malic acids, molecules in RNA synthesis GMP, AMP and inosine. In general, concentrations of most of these metabolites were low in J3 and J4 stages compared to egg, J2 and female which may be due to the sedentary nature of the nematode in those two stages. Proline was highly expressed in egg stage, muscle tone regulator GABA abundant in J2, whereas J3 and J4 stages possessed high concentrations of alanine and female was enriched with polyamine putrescine and amino acids.