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Helicobacter pylori is a Gram-negative, microaerophilic bacterium found in the stomach and causes chronic gastritis and gastric ulcers. It is also associated with the development of duodenal ulcers and stomach cancer. Therefore, sensitive detection of H. pylori is crucial for diagnosis and treatment of H. pylori associated diseases. It has been known that H. pylori expresses a sialic acid-binding adhesin (SabA) and a Leb-binding adhesin (BabA) that adhere, through glycan-adhesin interactions, to the human gastric mucosa for infection. To detect H. pyroli expressing BabA, we prepared fluorescent magnetic glyconanoparticles by conjugating aminoethylated Lea, Leband H1 oligosaccharides to carboxy-containing fluorescent magnetic nanoparticles. The successful preparation of glyconanoparticles was examined by zeta potential and transmission electron microscopy (TEM). When H. pyroli J99 strain which expresses BabA was incubated with three kinds of glyconanoparticles, fluorescence microscopy analysis showed that Leb and H1 conjugated but not Lea conjugated nanoparticles bound to this strain. However, these glyconanoparticles did not recognize H. pyroli strains lacking BabA. Finally, they were employed to enrich BabA expressing H. pyroli by using a magnet. It is expected that fluorescent magnetic glyconanoparticleswill be powerful tools to sensitively detect pathogens including H. pylori.