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Astragalin (kaempferol-3-O-D-glucopyranoside) is one of Flavonoid. Has been contain 0.12 percent of Astragalus sinicus and plant. However these plants only the least amount of Astragalin (kaempferol-3-O-d-glucopyranoside). By the aforementioned reasons, we are production of astragalin from kaempferol in Escherichia coli BL21. Kaempferol was further modified to produce astragalin by 3-O-glucosylation utilizing the endogeneous UDP-glucose in presence of UGT78K1 from Glycine max. E. coli strain was able to produce astragalin of conversion 70 percent per 0.4 mmol of kaempferol, A 50 milliliter of 200 millilter flask size culture. Grow in the shaking incubator for 35℃ 3hr, after check O.D 0.6 direct induction(1M IPTG) inject at the flask 20㎕ and shaking incubator for 20℃ overnight. But it is not maximum limit conversion of astragalin from kaemferol. We are add 0.1 percent glycerol of fed-bath culture, increase untill conversion 90 percent. In this work deliverable (conversion of astragalin) check of TLC and HPLC.