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The enzymatic reaction systems were developed for the efficient synthesis of glycoside products of arbutin, exists in two conformations, and . Beta-arbutin is often referred to as just arbutin. As a natural extract found in bearberry plants, arbutin also can infiltrate into the skin quickly without affecting the concentration of cell multiplication and effectively prevent activity of tyrosinase in the skin and the forming of melanin. Though arbutin is a natural derivative of hydroquinone, it does not possess the same risks or side effects. Arbutin has been shown to be a very safe ingredient and does not break down into hydroquionone very readily. The aim of the present study is to produce beta-arbutin by one-step reaction containing recycle systems, which is an enzyme reaction, not a chemical synthesis method. In an effort to produce UDP-glucose, we used specific enzyme, such as GalU, ACK, UMK, utilizing UMP as the substrates, and then beta-arbutin is synthesized through one-step reaction using YjiC, YdhE as a glycosyltransferase. The synthesized beta-arbutin was confirmed through the high-performance liquid chromatography (HPLC-PDA) and Mass, ¹H and ¹³C nuclear magnetic resonance (NMR). In excess of the substrate, the maximum productions of Hydroquinone glucosides (54% overall conversion of Hydroquinone) was achieved at 3 h incubation period. All the hydroquinone glycosides exhibited improved water solubility, diverse hydroquinone glycosides were produced in significant quantities by recycling UDP-a-D-glucose within one-pot in the glycosylation reactions.