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In this work, the specificity of glycosyl donors was studied to understand the material balance analysis on the products using a commercial CGTase. 4-hydroxyphenyl α-glucopyranoside (α-arbutin) and 4-hydroxyphenyl β-glucopyranoside (β-arbutin) were produced by transglycosylation reactions catalyzed by cyclodextrin glucanotransferase (CGTase) from sp.(Toruzyme®3.0L). The reactions were practiced in an aqueous system containing hydroquinone (HQ) and starch as acceptor and donor substrate molecules respectively. The conditions for the synthesis of hydroquinone glucoside (α-arbutinorβ-arbutin) were 20 mM hydroquinone, starch (3%,w/v) in pH5 and 0.04 mg/ml toruzyme at 75°C for 6 h. The transfer efficiency of hydroquinone glycosylation was 17% respectively, when starch was employed as donor substrates. The major glycoside product was identified as hydroquinone-1-O-d-glucopyranoside (arbutin) on the basis of mass spectrometric analysis. The highest molar yield of arbutin (17.0%) was obtained when starch was used as the donor substrate. At room temperature the solubility of arbutin in water was determined to be 12.8 g/100 ml which is approximately 1.8 fold higher than that of hydroquinone.