earticle

영어

Alzheimer's disease(AD), known as a chronic neurodegenerative disease, is the most common type of dementia with clinical symptoms. The pathogenesis of AD is highly believed to have a effect on the production and deposition of β-amyloid(Aβ) peptide. Swedish mutation or Familial Alzheimer’s disease genetic mutation is one of the most well known genetic variation that causes early onset familial AD. Gangliosides are key lipid molecules required for the regulation of cellular processes such as proliferation, differentiation and cell signaling. However the mechanism is not unclear between ganglioside and AD. This study was investigated relationship of gangliosides between ganglioside and AD. We made a cell line(WK-H4) using transformed Vector that is forced to gene over-expression of Aβ. We investigate the expression by using high performance thin-layer chromatograph(HPTLC), Western Blot(WB) and Immunofluoresence(IF). According to IF, the results show Aβ significantly increase in WK-H4. Also, HPTLC analysis shows that the expression of ganglioside GD1a is markedly diminished in WK-H4 than H4. Furthermore, β-Catenin at Wnt signal increases on WB analysis. While GSK-3β imperceptibly decrease in WK-H4. In conclusion, This study may reason out a conclusion that ganglioside is associated with AD and the selective control of ganglioside is helpful treatment of AD as a fresh bio-marker.