원문정보
초록
영어
Gangliosides are glycolipid having sialic acid. It is present in the central nervous system and involved in multiple functions of cell membranes and nerve function. However, roles of gangliosides in the differentiation of mesenchymal stem cells (MSCs) is unclear. Therefore, we investigated the effects of ganglioside in neuronal differentiation of adipose-derived MSCs (AD-MSCs). In the differentiated neuronal cells from AD-MSCs. we observed the expression of neuronal markers (MAP-2 and GFAP) and the high levels of mRNA expression of neuron-specific genes. Also, The expression of ganglioside GM2 and GD3 were significant increased in neuronal differentiation of AD-MSCs. In addition, this study showed that differentiation of AD-MSCs into neuronal cells significant decrease in the knockdown of UDP-glucose ceramide glucosyltransferase (UGCG) which well known as first step of ganglioside synthase and beta-1,4-N-acetyl-galactosaminyl transferase 1(B4GALNT1) gangllioside GM2 synthase. The results indicate that ganglioside GM2 and GD3 might be important roles in the neural differentiation of mini-pig AD-MSCs.