원문정보
초록
영어
Amylosucrase (ASase, EC. 2. 4. 1. 4) forms an insoluble α-1,4-glucan polymer by transferring glucose molecules liberated from sucrose to its appropriate substrates, such as maltooligosaccharides, arbutin, and salicin. It was found that ASase could be attached into the insoluble α-1,4-glucan polymer after its catalytic work was done. In this study, therefore, ASase, a glucan-polymerization enzyme, was employed as a fusion partner for foreign proteins to make the foreign proteins be easily purified. Green fluorescent protein (GFP) and β-glucosidase were used as model fusion partner proteins. ASase-foreign gene cassettes were constructed and transformed into E. coli (BL21 DE3). As a result, it was found that ASase-fusion proteins were easily purified by a simple centrifugation since the majority of ASase-fusion proteins were present in the insoluble glucan polymer. In this study, optimal condition of polymer production was characterized.