earticle

영어

T cells play a key role in the chronic inflammation and immune responses. We investigated the immuno-suppressive effects of glucosamine in both mice naive splenocyte and Jurkat T cell. Glucosamine inhibited T cell proliferation as well as IL-2, IFN-gamma, TNF-alpha induction in response to anti-CD3 or PMA/Ionomycin in splenocyte and Jurkat cell, respectively. MAP Kinase pathways play a critical role T cell activation and IL-2 production in T cell. Our data show that glucosamine inhibited PMA/Ionomycin induced JNK phosphorylation and anti-CD3 induced ERK1/2 phosphorylation in Jurkat cell. In addition, glucosamine suppressed nuclear factor of activated T cells (NFAT) transcription activity but not nuclear factor kappa B (NF-kB) and activator protein-1 (AP-1) of activated Jurkat cell. These results suggest that glucosamine might inhibit expressions of T cell-specific cytokines such as IL-2, IFN-gamma, and TNF-alpha via the NFAT pathway in activated T cells. These results demonstrate that glucosamine may be an important modulator of T-cell mediated immune response.