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Abstracts for Poster Presentation

Enzymatic Synthesis of Pure Maltohexaose from α-Cyclodextrin

원문정보

Phu Cuong Nguyen, Ji-Yun Kwak, Min-Jee Yoo, Jong-Tae Park, Kwan-Hwa Park

한국당과학회 한국당과학회 학술대회 2016 한국당과학회 동계학술대회 2016.01 pp.60-60
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초록

영어

Maltohexaose (G6) is a maltodextrin consisted of six-glucose in α-1,6-glycosidic linkages and essential research tool in food, cosmetic and pharmaceutical. In previous study, we have proven that the possibility of large-scale continuous production of pure maltodextrins from cyclodextrins (CDs) using Pyrococcus furiosus thermostable amylase with immobilization in a packed-bed reactor (PBR). In this study, we used this PBR for G6 production from α-CD. After enzyme reaction, 1-decanol was added to G6 mixture as precipitating agent with a 1:1 ratio of 1-decanol to α-CD. In which, the unreacted α-CD was precipitated, then it was separated by centrifugation. Finally, the G6-product was obtained in homogeneous form and high-purity (95 %). Through this study, we successfully demonstrated the enzymatic synthesis method to produce pure G6 from α-CD.

저자정보

  • Phu Cuong Nguyen Department of Food Science & Technique, Chungnam National University, College of agriculture & life sciences, Daejeon 305-764, Korea
  • Ji-Yun Kwak Department of Food Science & Technique, Chungnam National University, College of agriculture & life sciences, Daejeon 305-764, Korea
  • Min-Jee Yoo Department of Food Science & Technique, Chungnam National University, College of agriculture & life sciences, Daejeon 305-764, Korea
  • Jong-Tae Park Department of Food Science & Technique, Chungnam National University, College of agriculture & life sciences, Daejeon 305-764, Korea
  • Kwan-Hwa Park Department of Food Science & Biotechnology, Seoul National University, Seoul 151-921, Korea

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