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Abstracts for Poster Presentation

The mechanism and signal for the nuclear import of O-GlcNAc transferase

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영어

Uridine diphosphate-N-acetyl glucosamine, generated from glucose via the hexosamine biosynthetic pathway, is a substrate for O -linked-N-acetylglucosamine (O -GlcNAc). Addition and removal of O -linked-N-acetylglucosamine to Ser/Thr residues is a dynamic cycle and is involved in the regulation of nuclear and cytoplasmic proteins. Nucleocytoplasmic O -GlcNActransferase (OGT) attaches a single GlcNAc onto hydroxyl groups of serine and threonine residues. Although the cellular localization of OGT is important to regulate a variety of cellular processes, the molecular mechanism regulating the nuclear localization of OGT is unclear. Here, we characterized the NLS motif in OGT and this motif is required for the nuclear import of non-diffusible β -galactosidase. OGT bound the importin α5 protein, and this association was abolished when the NLS motif of ncOGT was mutated or deleted. We also revealed that O -GlcNAcylation on tetratricopeptide repeats (TPR) plays an important role in nuclear localization of OGT. Our finding suggests the mechanism behind how OGT can be localized in the nucleus and cytosol simultaneously.

저자정보

  • Hyeon Gyu Seo Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University
  • Han Byeol Kim Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University
  • Min Jueng Kang Department of Molecular Medicine and Biopharmaceutical Sciences, School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National University
  • Joo Hwan Rym Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University
  • Eugene C. Yi Department of Molecular Medicine and Biopharmaceutical Sciences, School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National University
  • Jin Won Cho Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University

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