earticle

영어

Understanding of the molecular relationships in carbohydrate-protein interactions provides useful information on biological processes in living organisms and is also helpful for development of potent diagonostic systems. Synthesizing the specific complex glycans is important for analysis of interactions between glycans and their binding proteins. Particularly, on-chip enzymatic synthesis of complex glycans can be an efficient and useful tool because this can directly provide diverse glycans to use for analyzing carbohydrate-protein interactions on the chip. We prepared novel on-chip glycan synthesis platform in which glycan-conjugated single-stranded DNA was synthesized using DNA synthesizer and the synthesized carbohydrate-oligonucleotide conjugates (COCs) were immobilized onto the complementary single-stranded DNA-modified glass slide. Due to the property of DNA to be possible for reversible hybridization and denaturation under certain conditions, we effectively recovered the synthesized glycans from the chip, and optimized the synthesis conditions for target complex glycans. We anticipate that this novel on-chip complex glycan synthesis can be successfully used in a number of glycan-related studies including analysis of diverse carbohydrate-protein interactions and high throughput diagnosis.