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The purpose of this study is to understand relations between dental hygienists’ subjective work environment and subjective pain symptoms in different regions and improve the environment of their treatment room and help them reduce pain in different areas. The subjects of this study are 250 dental hygienists. Data were collected from March until April, 2015 through a survey with self-administered questionnaire sheets, and the results of analysis are as written below. 1. Concerning the subjects’ general characteristics, 98.4% were females. About their age, 64.0% were ‘from 20 to 29’, and about years of service, ‘many years of service (over 6 years)’ formed 4.08%. About types of hospitals they worked for, ‘dental clinic’ formed the most as 7.08%, and about the department they worked for, ‘operative and prosthetic dentistry’ formed the biggest part as 30.4%. 2. About the subjects’ subjective health conditions, ‘I am healthy’ formed the biggest part as 39.6%, and about the areas of subjective pain, pain was felt the most in their neck as 19.4%, shoulders as 19.0%, waist as 17.5%, and legs as 16.3% in order. About ways to prevent or cure pain, ‘resting’ formed 33.2% and ‘no experience of being treated’ formed 21.6%, and about the degree of resting while working, ‘I rest sometimes’ formed 62.0%. 3. Regarding their subjective work environment, ‘physical environment’ received a low point as 2.89±0.51. About detailed items, they regarded their physical environment to be not very favorable giving low points to ‘There is little noise.’, ‘The treatment room is quite well equipped with ventilation facilities (windows or ventilators).’, and ‘An extra chair is equipped to each of the chair on the treatment room.’ in order. 4. Concerning their subjective pain symptoms in different regions, muscle symptoms received the lowest point as 2.40±0.78. About detailed items, ‘My shoulders are painful or uncomfortable.’ gained 2.20±0.92 points, and ‘My lower limbs (legs, knees, or feet) are painful or uncomfortable.’ received 2.37±1.00 points, and ‘My lumbar region (waist or back) is painful or uncomfortable.’ gained a low point as 2.38±0.97. 5. Regarding their subjective work environment and subjective pain symptoms in relation to general characteristics, there was no significant difference by years of service. About types of hospitals they worked for, dental clinics gained low points in ‘physical environment’, ‘the job system and environment’, and ‘muscle symptoms’, which indicated significant difference (p<0.05). About the degree of resting while working, ‘I hardly rest.’ was found to be low in ‘job environment and duties’, ‘skin symptoms’, ‘muscle symptoms’, and ‘psychological symptoms’, which indicated significant difference (p<0.05).